Bacterial & Yeast Growth | Bradford Assays | Battery Powered (rechargeable)
The OD600 DiluPhotometer™ is a small, easy-to-use instrument that is dedicated to measuring samples near the 600 nm wavelength. It is suitable for measuring growth rates of several cell types including E. coli and yeast. Use of the OD600 DiluPhotometer™ with Implen’s DiluCell™ 10 or DiluCell™ 20 facilitates the measurement of low volume samples (volume requirement of 200 µl and 100 µl respectively). The two different available DiluCell™ 10 and DiluCell™ 20 versions allow for automatic 1/10 and 1/20 dilutions. With a bandwidth of 40 nm, the OD600 is also suitable for protein quantification assays like Bradford (595 nm).
The instrument is battery operated and can be easily transported for usage in incubation cabinets and under anaerobic conditions. The batteries last for almost 1 month when fully charged.
The Lambert-Beer-Law, also known as Beer’s Law, empirically relates the absorption of light to the properties of the sample. This law states that there is a logarithmic relationship between the transmission of light through a specific sample (T = I/Io with I = outgoing light and Io = incoming light), the molar extinction coefficient for a specific compound (ε), the concentration of the absorbing species in the material (c) and the distance the light travels (d). By leaving all conditions constant but changing the pathlength (the distance the light travels), you can bend the Beer-Law to get an automatic dilution of your sample. This principle is realized within the OD600 DiluPhotometer™ with DiluCell™.
Protein Quantification with Bradford Assay
The Bradford Assay is a rapid and accurate method used to determine the total protein concentration of a sample by quantifying the binding of a dye, Coomassie Brilliant Blue G-250, to an unknown protein. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs. By comparing this binding to that of different, known concentrations of a standard protein, the concentration of the unknown protein can be calculated. Standard curves with bovine serum albumin (BSA) are given as an example.