Technical Notes

Technical Note: #8 OD600 The growth of bacteria in liquid culture media is commonly monitored by measuring the optical density at 600 nm (OD600). OD600 measurements are typically used to determine the stage of growth of a bacterial culture, these measurements help ensure that cells are harvested at an optimum point that corresponds… Download
Technical Note: #7 Flexible Unit Control The NanoPhotometer® Family offers a unique range of nanovolume and/or cuvette UV/VIS spectrophotometers with flexible control options. All NanoPhotometer® models can be accessed and controlled by a variety of control devices. Download
Technical Note: #6 Data Management The NanoPhotometer® family of UV/Vis spectrophotometers provides a well-structured data management portfolio to store different kinds of file formats in dedicated folders on different devices. Download
Technical Note: #5 Protein UV Performance Data Proteins, the dynamic parts of living cells, are involved in nearly all metabolic pathways of cells (e.g. enzymatic reactions, immune protection, and cellular response). The measurement of protein concentrations is frequently used in life science research. Although there are many assays available, care should be taken to select the optimum assay for the particular sample type…. Download
Technical Note: #4 Nucleic Acids Performance Data Nucleic Acid quantification is an important step in many different life science protocols including those implemented for NGS, qPCR and many other areas. The NanoPhotometer® offers a preprogrammed Nucleic Acid method with options to measure all types of nucleic acids like dsDNA, ssDNA, RNA, miRNA, Oligo, options to enter sequences for miRNAs and Oligos as well as a custom defined extinction coefficient for other types of nucleic acids. Download
Technical Note: #3 Blank Control The blank measurement as the first step for all photometric measurements is the most critical part for accurate and reproducible sample readings. It is a prerequisite for all photometric measurements. This Technical Note gives useful information about the blank solution, blank quality and information about the unique Blank Control™ feature in the NPOS software. Download
Technical Note: #2 Sample Compression Technology The patented Sample Compression Technology™ is key to obtain reliable and accurate readings from ultra-low volume samples. With the contained microenvironment geometry, the instrument is optimized for reliable performance in all climate zones and also is ideal for challenging studies Download
Technical Note: #1 True Path Technology The unique True Path Technology™ is the key component of the NanoPhotometer® for precise path length setting. It provides accurate and linear results over the lifetime of the instrument without any need for recalibration…. Download

Application Notes

Reading an Accurate Blank – The Most Important Measurement of the Day Thomas Sahiri. Munich, Germany Blanking an instrument is a frequent process carried out by researchers in a wet lab numerous times and yet – the comprehension of what reading a Blank on an Implen NanoPhotometer® or any other high quality UV/Vis spectrophotometer does and how easy the Blank reading can be compromised… Download
Measuring Protein Concentration in Cell Lysates by Direct UV280 Absorbance in the NanoPhotometer® P330 Renee Hirte, PhD Implen, Inc. Westlake Village, CA The most rapid and reproducible method to determine protein concentration over a wide range is by measuring absorbance at 280 nm by spectrophotometry. Accomplishing this task in the past has often been met with the challenge of working with limited volume… Download